The FluoroSpot assay combines the sensitivity of ELISpot with the capacity to study secretion of several analytes simultaneously, enabling studies of cell populations with different functional profiles. The FluoroSpot assay uses fluorescent detection instead of colorimetric. This allows for simultaneous detection of several analytes which is a major advantage, especially when cell numbers are limited. Detection of single analytes using FluoroSpot can be used as an alternative to regular ELISpot.
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